Effect of percoll density gradient centrifugation on motility, abnormality, and fertility in chicken spermatozoa.
نویسندگان
چکیده
منابع مشابه
Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution
Background: One of the factors limiting successful processing of alpaca (Vicugna pacos) semen is the viscosity of seminal plasma. The viscous nature of the collected ejaculate has hindered sperm cryopreservation as well as artificial insemination (AI) under field conditions. Aims: The objective of this investigation was to evaluate recovery, motility, ...
متن کاملTransmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa
The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before ...
متن کاملEvaluation of three substitutes for Percoll in sperm isolation by density gradient centrifugation.
Silane-coated silica particle solutions (ISolate(TM) and PureSperm)TM)) and iodixanol (OptiPrep(TM)) were compared to polyvinylpyrrolidone (PVP)-coated silica particles (Percoll(TM)) in their efficacy to recover spermatozoa by gradient centrifugation for use in assisted reproductive procedures. Efficacy was assessed in terms of percentages of sperm recovery, sperm vitality and motility, normal ...
متن کاملIsolation of spinach leaf peroxisomes in 0.25 molar sucrose solution by percoll density gradient centrifugation.
A procedure for isolating spinach (Spinacia oleracea L.) leaf peroxisomes in 0.25 molar sucrose solution by Percoll density gradient centrifugation followed by removal of the Percoll by washing and centrifugation was established. The preparation contains more than 90% peroxisomes as intact organelles with no detectable chlorophyll or cytochrome oxidase contamination. The peroxisomes are stable ...
متن کاملApplication of Percoll density gradient centrifugation for separation of Babesia ovata - infected erythrocytes
In this study, potential of the Percoll density gradient centrifugation method for separation of bovine red bold cells (RBCs) infected with each merozoite type of Babesia ovata was examined. By the Percoll density gradient centrifugation, bovine RBCs infected with active forms of B. ovata merozoites were essentially collected from one of the layers. Moreover, this method made it possible to eli...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese poultry science
سال: 1988
ISSN: 0029-0254
DOI: 10.2141/jpsa.25.237